A Novel Isoallergen Dau c 1.0401 in Carrot: Stability, Allergenicity, and Comparison with Other Isoallergens

Scope Around 25% of food allergic persons in Central Europe suffer from carrot allergy caused by the major allergen Dau c 1. Three different isoallergens, 1.01, 1.02 and 1.03 are identified. However, information about qualitative quantitative composition natural (n)Dau 1 is scarce. Methods Results The new 1.0401 identified on mRNA protein level RT-PCR mass spectrometry. It displays only around 60% sequence identity to other known isoallergens. NMR CD-spectra typical for a well-folded containing both α-helices β-strands. showed poor refolding capacity after incubation at 95 °C. IgE-binding impaired immunoblots, whereas inhibition assays IgE binding soluble detected it clearly provoked response mediator release assays. Conclusion isoallergen which contributes allergenicity carrots. absence immunoreactivity immobilized indicates that when blotted solid phase. Altogether, results point out its can be reduced upon processing.